Discovery Park Undergraduate Research Internship Program

"CPIP: Vitamin D and Breast Cancer Prevention"

About the Project

Project Time & Type:
Full-Year 2016 - CPIP
Research area(s):
vitamin D, breast cancer, metabolism
Project Description:
Vitamin D has been proposed to inhibit the progression of breast cancer and our laboratory is focused on understanding the mechanisms of this effect. In breast cancer progression cell energy metabolism, such that cancer cells have higher demand for glucose and glutamine to support rapid growth. Our laboratory has shown that vitamin D inhibits glutamine metabolism in the oncogene Harvey-ras transformed MCF10A human breast epithelial cells, a model of early breast cancer progression, which is regulated through inhibiting the expression of glutamine transporter, SLC1A5. The current project will focus on exploring if vitamin D has a similar impact in a more advanced, metastatic breast cancer cell line.
Expected Student Contributions:
The intern will have an independent project and will culture, treat and harvest mammalian cells and complete other appropriate analyses such as protein assays, Western blotting and rtPCR.
Related Website(s):
Desired Qualifications:
Previous experience in laboratory procedures and a background in topics related to biology are preferred.
Estimated Weekly Hours:
Department awards independent research credits for this project?
Yes, 3 credit hours

Professor in Charge

Teegarden, Dorothy
nutrition science

Student Supervisor

Dorothy Teegarden

Cooperating Faculty

Shawn Donkin
Animal Science, Agriculture