Discovery Park Undergraduate Research Internship Program

"CPIP: Identification of EMT markers in CRL1743 mammary cancer cells"

About the Project

Project Time & Type:
Full-Year 2014 - CPIP
Research area(s):
Cancer Prevention
Project Description:
Obesity is defined as a risk to health through the accumulation of fat that is excessive. Obesity has become a growing in problem, not only in America but in most parts of the world. The World Health Organization (WHO) has reported that obesity has nearly doubled since 1980 and that 65% of the world’s population live in countries where the mortality rate is high for those that are overweight and obese. Obesity has been associated with musculoskeletal disorders, cardiovascular diseases, diabetes, and some cancers. Breast cancer is the most common cancer in women (www.cancer.org) and cancer incidence has increased in younger women. Lifestyle factors such as physical activity and diet play a role in attenuating the incidence of breast cancer. Current research is starting to show that exercise during pregnancy is not only beneficial to the mother but can also convey a level of protectiveness to the offspring. Our lab is investigating the effects of exercise induced fetal programming in attenuating the incidence of turmorigenesis in MNU challenged rat pups.
Expected Student Contributions:
The student will analyze protein samples taken from a CRL1743 (ATCC) cell line. Techniques used will include cell lysis, BCA assays, Western blots, and Immunohistochemistry. Software will be utilized to quantify the degree of abundance of the protein of interest against a constitutively made protein. The student will be expected to read the literature pertaining to the project, perform presentations to the lab on the advancement of the project, and perform presentations outside the lab via Powerpoint and/or posters.
Related Website(s):
None
Desired Qualifications:
None
Estimated Weekly Hours:
10
Department awards independent research credits for this project?
Yes, 2 credit hours

Professor in Charge

Name:
Camarillo, Ignacio
Deptartment/College:
biological sciences

Student Supervisor

Name:
Ignacio
Title:
Associate Professor

Cooperating Faculty

Name:
Alice Chang
Deptartment/College:
Biomedical Sciences