Discovery Park Undergraduate Research Internship Program

"Cryo-electron microscopy image processing and 3-D reconstruction"

About the Project

Project Time & Type:
Fall 2013 - DURI
Research area(s):
structural biology; computational biology; image processing
Project Description:
Single particle electron cryo-microscopy and the associated image processing and 3-D reconstruction technique, collectively known as cryo-EM, has emerged in recent years as a powerful new method that allows biologists to study dynamic biological processes, such as virus assembly and virus-host interactions. In cryo-EM, the purified macromolecular particles are frozen and imaged in high-end electron microscope as 2-D projection images at unknown random views. Computational processing of the images is essential to determine the relative views of the different images termed 2-D alignment, and to reconstruct the 3-D structure of the virus by coherently merging large number of the 2-D images. This project will develop computational methods for high throughput image acquisition, processing, and 3-D reconstruction.
Expected Student Contributions:
The intern will research and implement algorithms to improve cryo-EM 3-D reconstructions. The improvements will target at higher resolutions, complex structure, or heterogeneous conformations. The intern will make a presentation in the lab meeting at the end of the semester.
Related Website(s):
Desired Qualifications:
programming (C/C++ or Python); math and physics; signal/image processing
Estimated Weekly Hours:
Department awards independent research credits for this project?
Yes, 3 credit hours

Professor in Charge

Jiang, Wen
biological sciences

Student Supervisor

Wen Jiang
Associate Professor

Cooperating Faculty

Charles Bouman
Electric and Computational Engineering