Discovery Park Undergraduate Research Internship Program

"Role of Adiponectin in Mammary Gland Tumorigenesis"

About the Project

Project Time & Type:
Spring 2013 - DURI
Research area(s):
obesity and breast cancer
Project Description:
Adiponectin is an insulin-sensitizing hormone that is secreted from adipocytes. Clinical studies have linked low adiponectin levels to increased risk of atherosclerosis, type II diabetes, metabolic syndrome and many forms of cancer. Subsequent studies have shown adiponectin has anti-atherogenic, anti-diabetic and anti-inflammatory effects. Recent evidence also reveals adiponectin inhibits proliferation of breast cancer and non-malignant mammary epithelial cell lines in vitro. As mammary development studies have helped define actions of hormones on epithelial behavior it has also become evident that many hormones critical for development are also important players in breast cancer. The objective of this study is to gain insight into the potential role of adiponectin signaling in the development of mammary gland tumors.
Expected Student Contributions:
Students will learn how to extract protein lysate from tissue collected from rats during tumorigenesis in the mammary gland that were either on a lean or high fat diet. Students will be asked to determine the quality of the samples collected through techniques such as the BCA assays. Students will use the protein lysate to run western blots to examine the expression of various genes.
Related Website(s):
None
Desired Qualifications:
None
Estimated Weekly Hours:
10
Department awards independent research credits for this project?
Yes, 2 credit hours

Professor in Charge

Name:
Camarillo, Ignacio
Deptartment/College:
biological sciences

Student Supervisor

Name:
Ignacio Camarillo
Title:
Associate Professor

Cooperating Faculty

Name:
Alice Chang
Deptartment/College:
Basic Medical Sciences