Discovery Park Undergraduate Research Internship Program

"Imaging miRNA expression in single cells"

About the Project

Project Time & Type:
Summer 2012 - DURI
Research area(s):
epigenetics, expression, nanotechnology
Project Description:
MicroRNAs (miRNAs) are short (22-25 nt) transcripts that bind ~6bp seed sequences on target mRNAs, usually leading to decreased target mRNA and protein levels. It is becoming increasingly clear that miRNAs drive gene regulation in many cellular processes and during differentiation, including pre-implantation development. You will be quantifying levels of miRNAs during embryonic development in single cells using nanotools.
Expected Student Contributions:
You will be collaborating with graduate students in developmental biology and nanotechnology to collect mouse embryos and use high-resolution microscopy and spectroscopy to assess miRNA expression in single cells. This will involve designing short oligos for detection and miRNA quantification.
Related Website(s):
http://www.ansc.purdue.edu/LossieLab/Welcome.html
Desired Qualifications:
I am looking for a student with a strong work ethic and good interpersonal skills who can think outside the box.
Estimated Weekly Hours:
40
Department awards independent research credits for this project?
No

Professor in Charge

Name:
Lossie, Amy
Deptartment/College:
animal sciences

Student Supervisor

Name:
Chiao-Ling Lo
Title:
Graduate Research Assistant

Cooperating Faculty

Name:
Joseph
Deptartment/College:
Irudayaraj