Discovery Park Undergraduate Research Internship Program

"Detecting kinase activity in leukemia cells using MRM mass spectrometry"

About the Project

Project Time & Type:
Spring 2012 - DURI
Research area(s):
biochemistry, diagnostics, leukemia, cancer, mass spectrometry
Project Description:
We are applying our peptide biosensor technology to test for kinase activity in leukemia cells, both from patients and from laboratory cell lines. We will be using multiple reaction monitoring (MRM) mass spectrometry to detect phosphorylation of our peptides after they have been incubated with live cells.
Expected Student Contributions:
The intern will assist with cell culture and peptide treatments, sample harvesting and preparation for MRM mass spectrometry analysis.
Related Website(s):
Desired Qualifications:
Students who have passed organic chemistry I and have experience with leukemia cell culture and human subjects research certification (CITI) will be preferred.
Estimated Weekly Hours:
Department awards independent research credits for this project?
Yes, 2 credit hours

Professor in Charge

Parker, Laurie
medicinal chem/molecular pharmacology

Student Supervisor

Steve Ouellette
graduate student

Cooperating Faculty

Hamid Sayar
IU Hematology/Oncology