Discovery Park Undergraduate Research Internship Program

"Role of Adiponectin in Mammary Gland Development"

About the Project

Project Time & Type:
Fall 2010 - DURI
Research area(s):
Cancer Research
Project Description:
Adiponectin is an insulin-sensitizing hormone that is secreted from adipocytes. Clinical studies have linked low adiponectin levels to increased risk of atherosclerosis, type II diabetes, metabolic syndrome and many forms of cancer. Subsequent studies have shown adiponectin has anti-atherogenic, anti-diabetic and anti-inflammatory effects. Recent evidence also reveals adiponectin inhibits proliferation of breast cancer and non-malignant mammary epithelial cell lines in vitro. As mammary development studies have helped define actions of hormones on epithelial behavior it has also become evident that many hormones critical for development are also important players in breast cancer. To date, there has been no examination of the role of adiponectin in non-malignant mammary epithelia in vivo. The objective of this study was to gain insight into the potential role of adiponectin in normal mammary gland development.
Expected Student Contributions:
The intern will be involved in isolating protein lysate from tissue taken from mice/rats at various stages of mammary gland development. Students will be asked to determine the quality of the samples collected through techniques such as the BCA assays. Students will use the protein lysate to run western blots to examine expression of various genes at the different stages.
Related Website(s):
Desired Qualifications:
Minimum GPA = 3.0; Must be able to work with animals
Estimated Weekly Hours:
Department awards independent research credits for this project?
Yes, 3 credit hours

Professor in Charge

Camarillo, Ignacio
Biological Sciences

Student Supervisor

Therese Salameh
Graduate Student

Cooperating Faculty

Kim Buhman
Foods and Nutrition