Discovery Park Undergraduate Research Internship Program

"CPIP: Plk1 in adaptive radioresistance of cancer cells"

About the Project

Project Time & Type:
Full-Year 2010 - CPIP
Research area(s):
Cancer biology
Project Description:
More than 50% of cancer patients receive radiotherapy at some points during the course of their treatment. One major challenge of radiotherapy is gradual acquisition of radioresistance of tumor cells, which is correlated to increased recurrence and failure in many patients. Thus, understanding the mechanisms of radioresistance of tumor cells is of clinically significance. Unfortunately, although radiation-induced genomic instability and stress-related pathways are known to modulate cell radiosensitivity, specific signaling molecules or networks regulating the adaptive radioresistance in tumor cells remain to be elucidated. Our recent preliminary data indicate that overexpression of Polo-like kinase 1 (Plk1), an important regular of many cell cycle-related events, is correlated with adaptive radioresistance of breast cancer MCF7 cells after fractionated irradiation (FIR). We propose to test the hypothesis that Plk1 might be a key player during FIR-induced adaptive radioresistance of tumor cells. Predicted results of the proposed studies will suggest that Plk1 inhibition might prevent acquisition of radioresistance of tumor cells, thus increasing efficacy of radiotherapy.
Expected Student Contributions:
The intern will perform the following experiments: --Radiation to generate radioresistant cell lines. --A lot of immunoblotting --Chromatin immunoprecipitation assays --Cell culture
Related Website(s):
http://www.ag.purdue.edu/biochem/Pages/liu8.aspx
Desired Qualifications:
GPA: 3.0 or above Special experience: working in a cell biology lab
Estimated Weekly Hours:
12
Department awards independent research credits for this project?
Yes, 4 credit hours

Professor in Charge

Name:
Liu, Xiaoqi
Deptartment/College:
biochemistry

Student Supervisor

Name:
Hongchang Li
Title:
Post-doc fellow

Cooperating Faculty

Name:
Chang-Deng Hu
Deptartment/College:
MCMP/Pharmacy