Discovery Park Undergraduate Research Internship Program

"REGULATION OF CELLULAR STRESS RESPONSE BY THE HUMAN FIC PROTEIN, HYPE."

About the Project

Project Time & Type:
Summer 2016 - DURI
Research area(s):
Biochemistry, Signal Transduction, Molecular Biology
Project Description:
During disease, such as cancer, cells experience various stresses that can eventually lead to cell death. To combat this stress, cells activate a protein signaling pathway called UPR (unfolded protein response). UPR activation initially functions to restore normalcy to the cell; however, upon sustained stress, UPR triggers programmed cell death, called apoptosis. Thus, UPR regulation is critical to determining cell fate. We have discovered that a human protein, HYPE, possesses the ability to chemically modify a chaperone called BiP, which serves as the sentinel protein to monitor UPR. HYPE belongs to a family of ‘Fic’ proteins that regulate various aspects of signal transduction across evolution. Modification of BiP by HYPE alters BiP function and UPR activation. Our goal is to understand the role HYPE plays in regulating this critical aspect of UPR regulation and determining cell fate. For the project to be conducted under the DURI program, we aim to study the parameters that govern the HYPE-BiP interaction, so as to develop therapeutics that could manipulate cellular stress response and prevent disease/tumor progression.
Expected Student Contributions:
We have generated structure models for the HYPE-BiP interaction. The intern will be involved in making point mutations in HYPE and BiP, followed by purification of these mutant proteins, and assessing the kinetics of interaction of mutants vs. wild type proteins.
Related Website(s):
Please see these two websites: https://www.bio.purdue.edu/People/faculty_dm/directory.php?refID=1079 http://www.purdue.edu/newsroom/releases/2015/Q2/protein-determines-life-or-death-fate-of-stressed-cells.html
Desired Qualifications:
1.GPA above 3.3 2. Coursework in Freshman Biology and Freshman Chemistry. Additional courses in Biochemistry, Cell Biology, and/or Organic Chemistry preferred but not required. 3. Prior molecular biology research experience in a lab, with good evaluation, required 4. Strong work ethic.
Estimated Weekly Hours:
40
Department awards independent research credits for this project?
No

Professor in Charge

Name:
Mattoo, Seema
Deptartment/College:
Biological Sciences

Student Supervisor

Name:
Anwesha Sanyal
Title:
Graduate Student

Cooperating Faculty

Name:
Jia Ma
Deptartment/College:
Bindley Biosciences - Biophysical Analysis
Name:
Tiago Sobreira
Deptartment/College:
Bindley Biosciences - Computational Biosciences