Discovery Park Undergraduate Research Internship Program

"Vitamin E tocotrienols as adjuvant therapy for colon cancer"

About the Project

Project Time & Type:
Full-Year 2017 - CPIP
Research area(s):
Cancer Prevention
Project Description:
Despite improved treatment and diagnosis, colorectal cancer (CRC) is still one of the leading causes of cancer death in the US. It is necessary to develop new and better therapeutic strategies to improve clinical outcomes. Recent studies suggest that nuclear factor (NF)-kB likely contributes to acquired resistance of 5-florouracil (5-FU) with or without oxaliplatin, a standard chemotherapeutic agent for CRC. Our pilot data indicate that vitamin E gamma- and delta-tocotrienol (gTE, dTE) potently inhibit NF-kB. We therefore hypothesize that addition of gTE or dTE to 5-FU/oxaliplatin will enhance 5-FU/oxaliplatin’s anticancer effect and therefore improve therapeutic outcomes. In this project, we will examine anticancer effects of combinations of gTE or dTE with 5-FU/oxaliplatin in human cancer cells. The underlying mechanisms will also be determined.
Expected Student Contributions:
The intern will have opportunity to perform cell culture studies and investigate the effect of tested compounds on cancer cell growth and viability using MTT assays. The intern will also have opportunity to conduct western blotting and flow cytometry to characterize indcution of apoptosis and other types of cell death. The participants may get invovled in ongoing animal studies that test anticancer efficacy in prelinical models.
Related Website(s):
http://www.purdue.edu/hhs/nutr/directory/index.php/profile/qjiang
Desired Qualifications:
The qualified students should have strong background in chemistry or biochemistry with GPA of 3.2 or above.
Estimated Weekly Hours:
9
Department awards independent research credits for this project?
Yes, 3 credit hours

Professor in Charge

Name:
Jiang, Qing
Deptartment/College:
nutrition science

Student Supervisor

Name:
Kilia Liu
Title:
graduate student

Cooperating Faculty

Name:
Yava Jones-Hall
Deptartment/College:
Comparative Pathology/Veterinary Medicine