Discovery Park Undergraduate Research Internship Program

"Cryo-electron microscopy data collection, image processing and 3-D reconstruction"

About the Project

Project Time & Type:
Fall 2015 - DURI
Research area(s):
instrument control; image processing; distributed computing; computational biology
Project Description:
Single particle electron cryo-microscopy and the associated image processing and 3-D reconstruction technique, collectively known as cryo-EM, has emerged in recent years as a powerful new method that allows biologists to study dynamic biological processes, such as virus assembly and protein translation by ribosome. In cryo-EM, the purified macromolecular particles are frozen and imaged in high-end electron microscope as 2-D projection images at unknown random views. Computational processing of the images is essential to determine the relative views of the different images termed 2-D alignment, and to reconstruct the 3-D structure of the structures by coherently merging large number of the 2-D images based on theory/method similar to that of medical CT scan. This project will develop computational methods for high throughput image acquisition, processing, and 3-D reconstruction.
Expected Student Contributions:
The intern will implement graphic interfaces to existing command line image processing programs, first for desktop computers and then as web service using Purdue DiaGrid. The intern will also research and implement ways of improving the underlying data collection, image process algorithms for higher quality 3-D reconstructions. The intern will attend weekly lab meeting and then make a presentation in the lab meeting at the end of the semester.
Related Website(s):
http://jiang.bio.purdue.edu/ http://diagrid.org/resources/cryoem http://cryoem.bio.purdue.edu
Desired Qualifications:
proficient in programming (C/C++ or Python); signal/image processing
Estimated Weekly Hours:
12
Department awards independent research credits for this project?
Yes, 4 credit hours

Professor in Charge

Name:
Jiang, Wen
Deptartment/College:
biological sciences

Student Supervisor

Name:
Wen Jiang
Title:
Associate Professor

Cooperating Faculty

Name:
Carol Song
Deptartment/College:
Rosen Center for Advanced Computing
Name:
Wen Jiang